| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: invention relates to the field of biotechnology, namely, to isolation of polyhydroxibutyrate (PHB) from dry biomass of microorganism obtained by fermentative synthesis. The method involves extracting dry biomass of microorganism of genus Azotobacter with content of PHB of 50 to 75%, that of nitrogen of 1.2 to 3.5% and ash of 10 to 25% by chloroform at temperature 30-40C and concentration of PHB in chloroform of 0.3 to 0.55 g/l, supplying PHB solution in chloroform to isopropyl alcohol evenly with rate of 1 to 3 l/hour with further separation and drying of target product. ^ EFFECT: obtaining a method of isolation of polyhydroxibutyrate for medicinal purpose with a preset molecular weight, output of 95-98.5% and purity of 99.5-99.95%, at process duration of 8-10.5 hours. ^ 1 tbl, 19 ex |
