摘要 |
The present invention provides human protein arrays that include at least 1000 human proteins. In another embodiment, the present invention provides a method for identifying a substrate of an enzyme, comprising contacting the enzyme with a positional addressable array comprising at least 100 proteins immobilized on functionalized glass surface, and identifying a protein on the positional addressable array that is bound and/or modified by the enzyme, wherein a binding or modifying of the protein by the enzyme indicates that the protein is a substrate for the enzyme. In additional embodiments, provided herein are methods for making an array of at least 1000 human proteins under non-denaturing conditions, including human proteins that are difficult to express and/or difficult to isolate in a non-denatured state.
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申请人 |
PROTOMETRIX, INC.;SCHWEITZER, BARRY;BALL, JAMES, A.;PREDKI, PAUL;MICHAUD, GREGORY, A.;ZHOU, FANG, X. |
发明人 |
SCHWEITZER, BARRY;BALL, JAMES, A.;PREDKI, PAUL;MICHAUD, GREGORY, A.;ZHOU, FANG, X. |