METOD OCH REAGENSROR FOR ENZYMIMMUNPROV

摘要

An enzymeimmunoassay method is disclosed wherein a reagent tube is used having at least its interior surface coated with an antiserum or antigen. A sample to be investigated or a standard solution is introduced in the reagent tube together with antigen- or antibody- complex labelled with an enzyme which enzyme uses pyridine nucleotide as coenzyme. The solution is reacted with the coating, and thereafter the solution is removed and the reagent tube is connected to a second tube containing a substrate solution for the label enzyme. The substrate solution is reacted with the coating of the reagent tube and the compounds attached to it. The connected tubes are turned to transfer the solution to the second tube whereupon the reagent tube is removed and a quantitative fluorometric assay is performed on the combined materials in the second tube. A shaped reagent tube for carrying out the method is also disclosed.