Fremgangsmåde til enzymatisk N-deacylering af 7beta-fenoxyacetamidocephalosporiner.

摘要

A process for the N-deacylation of a 7 beta -phenoxyacetamido cephalosporin which comprises subjecting the cephalosporin to the action of an amidohydrolase enzyme which is derived from a bacterial source, e.g. Erwinia aroideae, which is free from cells and deleterious enzymes, which is stabilised in solution by the presence of ferrous ions and which is effective to cause optimal N-deacylation at a pH of from 3.5 to 6.5.