| 摘要 |
<p>A diagnostic reagent holder specifically adapted for the detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection or by spectrophotometry. The holder comprises an elongate shaft with a support surface adjacent one end of the shaft. Diagnostic reagent is covalently attached to a self-supporting film such as a disc and firmly secured to the support. The disc may be retained in a groove in the support portion or may be adhered against the support surface. A projection or recess may be provided on the support to assist registry thereof with a cooperating portion of a viewing housing. A pivot extension projecting down from the holder forms a pivot point facilitating stirring. The diagnostic reagent, e.g., antibody, is stirred during incubation in contact with the sample to be detected, e.g., antigen, in solution. In a "sandwich" technique labelled substance (e.g., fluorochrome-labelled antibody) is reacted with the sample on the film followed by thorough washing. Then, the holder is slid into the novel viewing housing which precisely positions the film at a window for viewing as with a fluorometer. To avoid loss of labelled substance during handling, and especially during positioning in the viewing housing, one or more protective protrusions are provided around the periphery of the support surface.</p> |
