| 摘要 |
<p>It is known to serologically test for current or recent past gonorrhoeae infection by the reaction of antibodies in the sample with heat labile Neisseria gonorrhoeae (N.g.) antigen. However, such test procedures gave an undue number of high positive results arising from the reaction of other antibodies cross-reactive with N.g. The present invention overcomes this drawback by removing these cross-reactive antibodies prior to the addition of the N.g. antigen to the serum sample. Accordingly, the present invention provides a serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises heating the serum at about 56.degree.C. to 65.degree.C. for from about 15 to 30 minutes, the serum being undiluted or diluted with physiological saline at a dilution up to about 1:1000; adding the serum to about an equal volume of Neisseria meningitides sorbent composition containing from 0.1 to 100 sorbent units of Neisseria meningitides antigen to remove cross-reacting antibodies and thereafter mixing and incubating resulting mixture with a composition containing a heat labile Neisseria gonorrhoeae antigen produced from a culture of Neisseria gonorrhoeae antigen to form Neisseria gonorrhoeae antigen-antibody conjugate when said antibodies are present.</p> |
