摘要 |
Reagents for use in binding assays, particularly immuno assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including beta -galactosyl-umbelliferone-diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and omicron -phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3- omega -aminoalkyl and omicron -( omega -aminoalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to a beta -galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3- omega -carboxyalkyl and omicron -( omega -carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic polyamino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.
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