1,5-DIAZABICYCLO(3.3.0)OCTADIENEDIONE DERIVATIVES,PREPARATION AND USE THEREOF AS FLUORESCENT LABELLING AGENTS

摘要

Compounds of the formulas. (FORMULA) wherein R is independently branched or straight chain Cu-Cu alkyl; Ru is independently branched or straight chain Cu-Cu alkyl, phenyl or halo; Ru is independently branched or straight chain Cu-Cu alkyl wherein at least one Ru group has a bromo substituent on the carbon atom adjacent to the ring; and X is branched or straight chain Cu-Cu alkylene wherein the ring carbon and the quaternary nitrogen are attached to the same carbon atom are useful as chemical labeling agents which react readily with free sulfhydryl groups or primary amino groups to produce intensely fluorescent (when compounds I and III are used) and phosphorescent (when compounds II and IV are used) substances.