| 摘要 |
A method of isolating alpha1-fetoprotein sufficiently pure to be useful in radioimmunoassay procedures is described. The method comprises the procedures and techniques of affinity chromatography, blue dextran adsorption chromatography and polyacrylamide gel electrophoresis. Wherein a certain degree of reduction in kind and/or quantity of contaminants is not achieved by the first two procedures, gel filtration and mixed ion exchange cellulose chromatography are carried out in preparation for polyacrylamide gel electrophoresis. Monkey alpha1-fetoprotein very similar to human cord blood alpha1-fetoprotein by amino acid composition and physical properties and of sufficient purity to be useful in human radioimmunoassay procedures is obtained by the disclosed method.
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