| 摘要 |
PURPOSE:A specific lactic bacterium is cultured under specified conditions to produce glucan that can be used as starting material for artificial serum and carcinostatice agent. CONSTITUTION:A bacterium mold selected from Bacillus leaevolacticus M-4 (ATCC23549), the same M-1(ATCC23493) and the same M-91(FERM-544) is cultured in a medium containing 1-10wt/v% of sucrose as the carbon source, nitrogen source and other nutritions at 20-40 deg.C and a pH4-8 under standing or slight aeration to form and accumulate a polysaccharide that is of glucose polymer and entirely insoluble in water together with or solely a fructose polymer that has a molecular weight of over 20,000,000 and is soluble in water. They are, as it is or after slightly hydrolyzed with a mineral acid, isolated and purified. |
