| 摘要 |
PURPOSE:To produce the title substance effectively, by collecting creatine amidohydrolase from a culture of AK-2 strains which belongs to the genus Alcaligenes. CONSTITUTION:Strains of microorganisms AK-2 which belong to the genus Alcaligenes are cultured in a medium comprising creatine and/or creatinine as a main nitrogen and carbon sources, and an inorganic base, e.g. ammonium chloride or sulfate and an organic nutriment in a trace amount, e.g. yeast or malt extract, at about a neutral pH and 25-35 deg.C for 1-2days aerobically with stirring to propagate the bacterium bodies wherein the enzyme is accumulated. The enzyme is then extracted from the bodies, separated, and purified. EFFECT:Creatinine amidohydrolase and creatine amidinohydrolase can be produced at an enzyme activity ratio of 6:1-1:2. |
