| 摘要 |
<p>Displacement electrophoresis is carried out on a gel (11) having the form of a tube along which substances are separated in the axial direction. The method makes use of an agarose or polyacrylamide gel (11) formed on and supported by an inner tube (12) which also acts as a conduit for coolant. Very thin gels are used to provide a short path for heat removal e.g. up to 3 mm. The gel is supported on one side only and the other side is in contact with electrolytes used in the process i.e. leading, displacing, and terminating electrolytes. To avoid drying out of the thin gel it is maintained in contact with an inert water-immiscible liquid where it is not in contact with electrolyte. The method is of especial importance for the separation of ampholyte molecules such as proteins or peptides. To provide an indication of the extent of separation fluorescent ampholytes are used as marker substances in very small quantities. </p> |
