发明名称 |
PROCESS FOR ISOLATING A SPECIFIC NUCLEOTIDE SEQUENCE CONTAINING GENETIC INFORMATION AND TRANSFER TO A MICROORGANISM |
摘要 |
New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.
|
申请公布号 |
PT68082(B) |
申请公布日期 |
1980.02.20 |
申请号 |
PT19780068082 |
申请日期 |
1978.05.24 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
WILLIAM J. RUTTER;HOWARD MICHAEL GOODMAN;AXEL ULLRICH;JOHN SHINE;JOHN MITCHELL CHIRGWIN;RAYMOND LOUIS PICTET;PETER HORST SEEBURG |
分类号 |
C12N15/09;C07K14/575;C07K14/62;C12N1/00;C12N1/16;C12N1/21;C12N15/00;C12N15/10;C12P19/34;C12P21/00;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|