摘要 |
Methods for packing chromatographic columns are disclosed, including introducing a packing material into a chromatographic column under the influence of a first pressure differential until the column is filled to a desired extent with the packing material, and subsequently establishing a substantially regular periodic vibration along the length of the column and subjecting the packing material to a second pressure differential, which is preferably greater than the first pressure differential. The substantially regular periodic vibration can comprise ultrasonic vibration, which is preferably applied to the column by immersing it in a liquid bath and subjecting the liquid bath to ultrasonic vibrations. |