| 摘要 |
PURPOSE:To purify pancreatic kallikrein useful as circulatory medicines, having a peripheral vasodilator action, by adsorbing crude kallikrein on a specific reagent selectively. CONSTITUTION:A crude kallikrein solution containing enzymes and different proteins is brought into contact with a p-amino-benzamidine bonding carrier to adsorb kallikrein (K) selectively. K is then eluted to purify the pancreatic K. A combination of carrier-(e.g. agarose activated by cyanogen bromide)-spacer (e.g. epsilon-amino- caproic acid)-p-amino-benzamidine) is preferred as the carrier, and usable repeatedly.
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