| 摘要 |
<p>A method is disclosed whereby the molecules of a chemical substance to be measured, referred to as target molecules, are combined with fluorescent molecules having a relatively long fluorescent decay lifetime. These tagged target molecules are excited with an intense pulse of ultraviolet or visible radiation, and a detection system is gated on only after background fluorescence has substantially decayed, but while the fluorescent tag attached to the target molecules is still actively decaying. The amount of fluorescent tag emission which is measured is indicative of the target molecule content of the sample. An apparatus for improved fluorescent spectrofluorometry includes a pulsed excitation source and a gating means connected to a detection system for gating the detection system on after tagged target molecules have been excited and competing background fluorescence has substantially decayed, but before tagged target molecules have substantially decayed.</p> |
