发明名称 |
ATTENUATED SMALLPOX VACCINE STRAIN |
摘要 |
<p>The invention provides an attenuated smallpox vaccine strain which exhibits no invasiveness caused by peripheral infection on the central nervous system of a mouse, low neuropathogenicity in Cynomolgus monkey, and little postvaccinal side effects. The strain is prepared by subculturing a Lister strain in mammal kidney cells at low temperatures, plaque-purifying the strain thrice, selecting a temperature-sensitive variant, subculturing the temperature-sensitive variant in mammal kidney cells, plaque-purifying the variant twice to isolate a clone showing relatively small and uniform pocks on the chorioallantoic membrane of an embryonated egg, subculturing the clone in mammal kidney cells and isolating a clone showing very small pocks on the chorioallantoic membrane of an embryonated egg.</p> |
申请公布号 |
EP0157528(A3) |
申请公布日期 |
1986.12.30 |
申请号 |
EP19850301859 |
申请日期 |
1985.03.18 |
申请人 |
CHIBA SERUM INSTITUTE, (OF CHIBA PREFECTURAL GOVERNMENT) |
发明人 |
OOI, KIYOSHI;MORITA, MICHIO;SUZUKI, KAZUYOSHI;HASHIZUME, SOH;YOSHIZAWA, HANAKO |
分类号 |
A61K39/285;C12N7/08;(IPC1-7):C12N7/08 |
主分类号 |
A61K39/285 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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