ANTI-CHONDROCALCIN-MONOCLONAL ANTIBODY, HYBRIDOMA PRODUCING SAME AND ITS PRODUCTION

摘要

PURPOSE:To produce a monoclonal antibody recognizing mammal-,esp.human- chondrocalcin by culture of hybridoma producing the monoclonal antibody to mammalian chondrocalcin. CONSTITUTION:A mouse is immunized with the chondrocalcin of mammalian origin followed by fusion of the lymphocytes of this mouse with mouse myeloma cells to produce a hybridoma. Since this hybridoma is capable of producing various monoclonal antibodies corresponding, respectively, to various lymphocytes fused, a cloned hybridoma is isolated by aimed monocloning. This cloned hybridoma is then put to in vitro culture to secrete monoclonal antibody. The objective anti-chondrocalcin-monoclonal antibody is then isolated from the resultant culture supernatant.