| 摘要 |
PURPOSE:A microorganism belonging to Brevibacterium or Corynebacterium, which produces beta-hydroxy-beta-carboxy-isocaproic acid, is cultured and title acid used for rust preventive or chelating agent is extracted from the medium. CONSTITUTION:A microorganism belonging to Brevibacterium or Corynebacterium, which is capable of producing beta-hydroxy-beta-carboxy-isocaproic acid, such as Brevibacterium flavum AJ 11230 (FERM-P4397: beta-hydroxyleucine-resistant mutant) or Corynebacterium acetoacidiphilum AJ 11231 (FERM-P4398: leucine-requiring mutant) is aerobically cultured in an enriched medium at a pH of 4-8 and a temperature of 25-38 deg.C for 1-7 days and title acid that is accumulated in the medium is extracted with a solvent and isolated through the ion-exchange method or the like. |
