发明名称 |
Curculin B and DNA encoding same, and process for production thereof |
摘要 |
Essentially pure curculin B is disclosed. A process for producing curculin B, includes the steps of: culturing a transformed cell or microorganism containing a recombinant DNA containing a base sequence encoding curculin B, whereby curculin B is produced by the transformed cell or microorganism, and isolating curculin B from the transformed cell or microorganism. A DNA encoding mature or premature curculin B is also disclosed. DNA including a base sequence encoding curculin B, is produced by: separating a fraction containing a curculin B mRNA from Curculigo latifolia, preparing a single-stranded DNA from the mRNA using reverse transcriptase, preparing a double-stranded DNA from the single-stranded DNA, inserting the double-stranded DNA into a vector, transforming a host with the vector to produce a cDNA library, and isolating a cDNA encoding curculin B from the library, using one or more synthesized DNA's containing a base sequence coding for a partial amino acid sequence elucidated from curculin A purified from Curculigo latifolia, as a probe.
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申请公布号 |
US5480795(A) |
申请公布日期 |
1996.01.02 |
申请号 |
US19920845592 |
申请日期 |
1992.03.04 |
申请人 |
KURIHARA, YOSHIE;ARAI, SOICHI;ASAHI DENKA KOGYO K.K. |
发明人 |
KURIHARA, YOSHIE;ARAI, SOICHI;ABE, KEIKO;YAMASHITA, HARUYUKI |
分类号 |
C07K14/245;A23L1/227;A23L1/236;A61K38/00;C07K14/43;C12N15/29;C12P21/02;(IPC1-7):C12N15/29;C12P19/34 |
主分类号 |
C07K14/245 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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