| 摘要 |
<p>PURPOSE:To provide the title culture method in such a manner that hepatocytes are put to culture under specified conditions, and using the resultant culture solution separated, hepatic cells are subjected to culture for a long period in such a state as to be suspended in a spherically aggregated form, while retaining stabilized functions and form. CONSTITUTION:Hepatocytes are put to culture in a negative electric charge-free plastic container, using a serumless medium absent from any substrate for cell adhesion and interstitial matter out of hepatic cell, and the resultant cells are separated by, e.g., ultrafiltration to prepare a culture solution. Thence, a spherical cluster of hepatic cell is added to said culture solution to make a culture of the hepatic cells.</p> |
