| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new type II restriction enzyme specifically recognizing a new base sequence in a double stranded deoxyribonucleic acid, specifically cleaving the same and useful in a field of gene technology such as an analysis of a huge DNA by clouting a specific strain belonging to the genus Streptomyces. SOLUTION: This restriction enzyme is obtained by culturing Streptomeyces-sp. RH 232 (FERM-P-15725), etc., specifically recognized a base sequence of the formula (G is guanine; C is cytosine) in a double stranded deoxyribonucleic acid and specifically cleaves the same at a position marked by an arrow. The restriction enzyme Sse-232I produced by the Streptomyces-sp. RH 232, has the following physicochemical properties: (1) an optimum temperature is approximately at 37 deg.C; (2) an optimum pH range is 7.5-8.0; (3) molecular weight thereof is approximately 60,000-90,000 (by an equilibrium density gradient centrifugal separation method). |
