Accellular synthesis of cephalosporins

摘要

A cephalosporin antibiotic is produced from a precursor comprising the five membered thiazolidine ring and the beta lactam moiety characteristics of penicillins by contacting the precursor with a cell-free extract of Cephalosporium acremonium in the presence of ATP under conditions favoring high oxygen transfer. Preferably, an ATP regeneration system comprising a phosphate donor and a phosphotransferase enzyme is included in the reaction.