发明名称 |
SYSTEMS AND METHODS FOR DETERMINING AN AMOUNT OF STARTING REAGENT USING THE POLYMERASE CHAIN REACTION |
摘要 |
<p>Systems and methods for calculating an initial amount of target nucleic acid N0 in a sample are provided. A plurality of fluorescent measurements is received. Each respective fluorescent measurement FSn is taken in a different cycle n in a PCR amplification experiment of the sample. Then, a model for the PCR amplification experiment is computed. For each respective fluorescent measurement, the model comprises a respective equation for Nn, where (i) Nn is the calculated amount of the target nucleic acid in cycle n of the corresponding PCR amplification experiment, and (ii) the equation for Nn is expressed in terms of K and N0, where K is the Michaelis-Menton constant. The model can be refined by adjusting K and N0 until differences between model values Nn and corresponding fluorescent measurements are minimized, thereby calculating the initial amount of a target nucleic acid N0 as the minimized value for N0 for the model.</p> |
申请公布号 |
WO2009029710(A1) |
申请公布日期 |
2009.03.05 |
申请号 |
WO2008US74614 |
申请日期 |
2008.08.28 |
申请人 |
BECTON, DICKINSON AND COMPANY;BARTKOWIAK, MIROSLAW;MOORE, RICHARD L. |
发明人 |
BARTKOWIAK, MIROSLAW;MOORE, RICHARD L. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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