THF PREPARATION

摘要

Chemically substantially pure and uniform THF (thymic hormone), substantially devoid of endotoxins, having a molecular weight of 3323? 220, an isoelectric point of form 5.7 to 5?9; an amino acid composition of approximately: aspartic acid - 4 units; threonine - 1 unit; serine - 5 units; glutamic acid - 8 units; praline - 2 units; glycine 5 units; alanine - 2 units; leucine - 1 unit; lysine - 1 unit; arginine 2 units; ? cystine - 1 to 2 units; being the product of purification of thymus tissue ant removal there from of endotoxins, and a process for the preparation of same which comprisesa) comminuting fresh thymus tissue from mammals in a suitable liquid medium; at a pH between 7 and 8;b) removing cell debris and other cell residues by centrifugation and ultracentrifugation;c) separating the supernatant liquid and filtering same through suitable filtration media to remove particulate material and so as to sterilize the passing liquid ant to remove endotoxins,d) subjecting the liquid to exhaustive dialysis;e) lyophilizing the dialyzate and redissolving it in a suitable liquid medium;f) subjecting the lyophilized redissolved material to gel filtration and assaying the resulting peaks (fractions), andg) eluting the active constituent in substantially pure form; and pharmaceutical compositions of matter containing same as active ingredient and a process of immunorestorative therapy of mammals which comprises administering to the mammal a therapeutically effective dose of such THF.