Method for sized-based cell separation using spinning membrane filtration

摘要

A method is provided for separating a suspension of cellular material comprising at least two differently-sized cell types using a spinning membrane separator. The method comprises selecting the cell type to be separated by passing through the membrane; determining a concentration of the selected cell type in the suspension; selecting an inlet flow rate for the suspension; selecting a rotational speed for the spinning membrane separator related to one or more of the concentration and relative size of the selected cell type in the suspension; rotating the spinning membrane separator at the selected rotational speed so that the selected cell type tends to migrate to regions of the shear field adjacent the porous membrane; and flowing the suspension through the spinning membrane separator.