SEQUENCE SPECIFIC ACID PROTEASE OF E. COLI

摘要

An acid protease of E.coli which catalyzes cleavage between a) adjacent lysine sequences, b) adjacent arginine sequences, c) lysine-arginine sequences and d) arginine-lysine sequences in proteins is disclosed. Also disclosed is an E.coli protein expression vector comprising the following operably linked genetic components: i) a cytochrome c peroxidase gene; ii) an E.coli promoter; iii) an E.coli origin of replication; and iv) an E.coli drug resistant gene. The vector induces production of the protease in E.coli.