| 摘要 |
PURPOSE:To prepare immobilized microorganisms having high stablity and catalytic activity, composed of a gel covered with a layer of a desired thickness containing living microorganisms in high concentration, easily (without using mass culturing), by the use of a particular inclusion technique. CONSTITUTION:Pellet or membrane of gel having a thickness of 2-50 mm and containing 0.01-10 oese of living microbial cells (based on 100 g (wet basis) of gel) is prepared, e.g. by adding the cells or the culturing medium containing the cells to an aqueous solution containing agar, etc. gelling the solution using a gelling agent, etc., and forming the gel to the desired form. The gel is incubated in a medium suitable for the growth of the microorganism at 25-40 deg.C to obtain the layer of the living microbial cells near the gate surface. The thickness of the layer can be controlled by controlling the culturing conditions. |
