PROCESS AND COMPOSITION FOR DETERMINING THE ACTIVITY OF CREATINEKINASE-MB

摘要

A method, composition and test kit are provided for determination of CK-MB activity in a sample of biological liquids. The method comprises the steps of incubating the sample, optionally in the presence of CK substrates, with antibodies which inhibit enzymatic activity of the M subunit in CX isoenzymes MM and MB without substantially inactivating enzymatic activity of the B subunit in any CK-MB which may be present, and then determining the activity of the CK B subunit in the resultant sample. This provides a simple, rapid and reproducible method for the diagnosis of cardiac infarction.