IMPROVEMENTS IN OR RELATING TO ANTIBIOTICS

摘要

1479063 Antibiotics PFIZER Inc 16 April 1975 [16 April 1974 17 Jan 1975] 15748/75 Heading C2A A synergistic antibiotic mixture comprises compounds 35, 763, 36, 926, 37, 277 and 37, 932 and miscellaneous lesser antibiotic components produced by cultivating Actinoplanes auranticolor ATCC 31011 under submerged aerobic conditions in an aqueous nutrient medium containing an assimilable source of carbon and nitrogen until substantial antibiotic activity is obtained and separating the antibiotic mixture therefrom. Antibiotic compound 37, 277 is in crystalline form and has an optical rotation of [α] D 25‹= + 11‹ at a concentration of 1% in ethanol, absorption maxima in ethanol in the ultra-violet region at 225, 274, 282, 303 and 355 mÁ with E1% 1cm values of 309À3, 36.67, 45.01, 70 and 20, an average composition by weight of 60.91% C, 5.98% H, 10.45% N and 22.66% O, when dissolved in chloroform exhibits the absorption characteristics indicated in Fig. 1 (not shown), on heating decomposes between 145‹ and 150‹ C., is insoluble in diethyl ether, Lexane, treptane and water, slightly soluble in acetone and leuzene, and readily soluble in methanol ethanol, chloroform and methylene chloride. Antibiotic compound 36, 926 has an average composition by weight of 57.89% C, 6.78% H, 8.04% N and 27.29% O, a molecular formula C 26 H 35 N 3 0 7 , an optical rotation of [α] D 25‹=-130‹ at a concentration of 1% in ethanol, absorption maximum in ethanol in the ultra-violet region of the spectrum at 214 mÁ. with E1% 1cm of 723À8, 8 when spectrum at 214 mÁ with E1% 1cm of 723À8, when pelleted on KBr exhibits the absorption characteristics indicated in Fig. 2 (not shown), and on heating, decomposition commences at 100‹ C., is soluble in ethanol, methanol, chloroform and methylene chloride and insoluble in diethyl ether, hexane and heptane. Antibiotic compound 35, 763 has the molecular formula C 26 H 27 N 3 O 7 , an optical rotation of [α] D 25‹=-114‹ at a concentration of 1% in ethanol, absorption maximum in ethanol in the ultra violet region at 218 mÁ with E1% 1cm of 668.9, when pelleted in KBr exhibits the absorption characteristic indicated in Fig. 3 (not shown), is soluble in methanol, ethanol, chloroform and methylene chloride, insouble in diethyl ether, heptane and hexane, on heating decomposition commences at 100‹ C., has a M.Wt of 503 and an average composition by weight of 61À29% C, 6À73% H, 8À83% N and 23À15% O. Antibiotic compound 37, 932 is in crystalline form and has an optical rotation of [α] D 25‹ = + 5.0‹ at a concentration of 0.25% in chloroform, absorption maxima in ethanol in the ultra-violet region of the spectrum at 226, 276, 283, 305 and 355 mÁ with E1% 1cm values of 304.4, 36.8, 43.49, 70.25 and 20.07, an average composition by weight of 59.41% C, 6À01% H, 10À66% N and 23.92% O when pelleted in KBr exhibits the absorption characteristics indicated on Fig. 4 (not shown) and is soluble in methanol, ethanol, chloroform and methylene chloride, is insoluble in diethyl ether, hexane, heptane and water and on heating decomposition commences at 185‹ C. The antibiotic mixture or one or more of the antibiotics may be admixed with a pharmaceutically acceptable carrier to form a pharmaceutical composition. The antibiotic mixture or one or more of the antibiotics may be incorporated in an animal feed composition.