Method for insolubilizing enzymes on chitosan

摘要

Insolubilized but active enzymes are prepared in a variety of ways. In one embodiment of the invention an aqueous solution of an enzyme is mixed with chitosan dissolved in water at pH 3-7 and an insolubilized product is precipitated therefrom by addition of alkali or a source of sulfate ions. Another embodiment of the invention involves contacting solid chitosan sulfate with an aqueous solution of the enzyme to be immobilized. In yet another embodiment of the invention solid chitosan is cross-linked with a polyfunctional cross-linking agent and then contacted with an aqueous solution of an enzyme. Enzymes insolubilized in accordance with the invention retain a substantial part of their original activity.