METHOD AND APPARATUS FOR CHROMATOGRAPHIC SEPARATING MIXTURES CONSIST OF SEVERAL MATERIALS

摘要

1295386 Continuous cyclic chromatography BOEHRINGER MANNHEIM GmbH 19 July 1971 [23 July 1970 13 March 1971] 33784/71. Heading B1X A process for the continuous cyclic chromatographic separation of a multicomponent mixture utilising two columns connectable in series and which may be packed with ion exchangers comprises alternately passing the mixture and element, e.g. H 2 O to the first column 3 from sources S and W respectively by means of dosing pumps 1, 4 and switching clock 6. Eluent then passes to the second column 10. Any predetermined undesirable fraction emerging from the first column is detected by a detector 7, e.g. a flow through refractometer, which causes control 12 to switch a valve 8 to its Fig. 1 position so that the fraction is diverted to drain, and the connection between the columns is broken. During this time H 2 O is supplied to the second column 10 via pump 9. The fractions emerging from the second column are detected by detector 13, e.g. for measuring angle of rotation or refractive index, and diverted by valve 15 and its control 14 to the appropriate vessels. The columns may be packed with polystyrene sulphonate resin cross-linked with divinylbenzene and loaded with calcium ions. The multicomponent mixtures may be epimerised or non-epimerised starch syrup, or a salt free solution of starch hydrolysate which are to be split into glucose and fructose, and the impurities may be oligosaccharides.