| 摘要 |
PURPOSE:To prepare a high ribostamycin by the aerobical cultivation of specific mutant belonging to Bacillus circulans in a nutrient medium. CONSTITUTION:A mutant of Bacillus circulans, B-15M451 (FERM-P No.4320) obtained by the nitrosoguanidine-treatment of Bacillus circulans B-15M-306 which is known ribostamycin-producing bacterium, is inoculated in a nutrient medium, and cultured at pH5.5-8.5 and 20-40 deg.C in an aerated deep-tank fermentor. A large amount of ribostamycin free from xylostasin is accumulated in the medium, and is easily separated from the medium by the extraction with an organic solvent or by the chromatographic treatment with an adsorbent. |
