Determination of blood heparin concn. - by rapid, sensitive and accurate method using heparin as anticoagulant

摘要

Blood heparin concns. in heparinised patients are determined by adding serum from the blood to standardised thrombin soln. and incubating the mixt. at a selected temp. for a defined period (too short for reaction between thrombin and any fibrin degradation prod. but sufficient for reaction between thrombin and heparin). A standard portion of the mixture is then added to normal plasma that has been equilibrated at the same selected temp. The clotting time of the resulting mixture is observed; it is an inverse function of the amt. of thrombin left after reaction with heparin and is a direct function of blood heparin. The clotting time is compared with standard curves prepd. by using serum from healthy individuals to whch standard amts. of heparin had been added. Rapid, sensitive and reliable method for determining heparin in blood to give accurate monitoring when heparin is used as an anticoagulant. The relationship between heparin content and the concn. of antithrombin III can be drawn.