| 摘要 |
<p>A new surface immunoassay method is performed with two test surfaces. The method is used where serum samples may contain both an antibody for which it is desirable to test, and also competing sera constituents which may be present in indeterminate amounts. In accordance with the method, two different test surfaces are contacted with the same serum sample, and preferably the same aliquot of the serum. The first surface is treated to bind a broad spectrum of serum components including both the component to be tested for and also the competing components. The second surface is designed to bind the competing components but no substantial amount of the serum component to be tested for. The immunoassay is conducted with a labeled antibody, and quantitative measurement of the immunoassay is obtained by detecting the quantitative difference in the amount of labeled antibody on the two surfaces which have been subjected to the same serum sample. The method has utility in detecting a wide variety of antibodies of both the immunoglobulin G and immunoglobulin M classes, including antibodies for rubella, treponema, herpes simplex virus, cytomegalovirus and toxoplasma. Preferably the two surfaces used in the test are mounted on a single sampler which is analyzed in the FIAX TM Fluorometer.</p> |
