METHODS OF USING TRICHLORO-S-TRIAZINE FOR COUPLING ENZYMESAND AFFINITY LIGANDS TO SOLID-PHASE SUPPORTS

摘要

Proteins and non-protein affinity ligands are covalently bonded to trichloro-s-triazine activated supports. The activated support is prepared by reacting a water-free insoluble solid support with trichloro-s-triazine in a non-aqueous medium and neutralizing HCl generated during the reaction with a tertiary amine which does not form an insoluble complex with the trichloro-s-triazine.