| 摘要 |
PURPOSE:To obtain accurate values by correcting the measured values of samples by the use of the values of chromogen quantities obtained through measurement in the region where the samples do not have absorption in the measurement of the samples containing disturbing chromogen by a multi-item colorimetric analysis method. CONSTITUTION:For example, in analysis of samples containing chromogen of 3 components respectively indicated by standard solution spectra of chyme 20, hemolysis 22 and jaundice 24, the above region refers to the region where the intended substance does not have absorption. For example, from the difference in absorbancies of lambda20 and lambda21, lambda18 and lambda19, lambda15 and lambda16, the degree of tchyme X, the degree of hemolysis Y and the degree of jaundice Z are respectively obtained by using the formulas (1), (2), (3) (where, An-m is absorbancy difference between lambdan and lambdam; Tn-m is the unit absorbancy difference per unit of degree of specimen between lambdan and lambdam; Hn-m is the absorbancy difference per unit degree of hemolysis at lambdam; Bn-m is the absorbancy difference per unit degree of jaundice at lambdam) and from these values, the value S having been obtained through the colorimetiric analysis of the sample is corrected by the use of the formula (4) (alpha, beta, gamma are conversion coefficients), whereby the correct analytical value S<1> is obtained. |
