发明名称 |
Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes |
摘要 |
Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.
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申请公布号 |
US4155916(A) |
申请公布日期 |
1979.05.22 |
申请号 |
US19770828394 |
申请日期 |
1977.08.29 |
申请人 |
DOLBEARE, FRANK A;SMITH, ROBERT E |
发明人 |
DOLBEARE, FRANK A;SMITH, ROBERT E |
分类号 |
C07D311/20;C12Q1/00;(IPC1-7):C07C119/00 |
主分类号 |
C07D311/20 |
代理机构 |
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