Process and apparatus for pulse labelling protein material in the Edman degradation process

摘要

A process and apparatus for pulse labelling protein material for high sensitivitiy sequence analysis of protein material by Edman degradation technique comprising an isolated, auxiliary unit for storing a radioactive coupling reagent together with tubes, valves and controls for interrupting the Edman process, for pumping a measure of radioactive coupling reagent into the reaction vessel, a timer for controlling duration of contact between the radioactive reagent, and the protein material to maximize the coupling reaction therebetween, and thereafter reactivating the Edman process to admit nonradioactive coupling reagent to the vessel and to drive the coupling reaction to completion.