发明名称 |
Method and apparatus for the continuous determination of the concentration of an enzyme substrate |
摘要 |
Enzyme substrates in aqueous liquids are continuously measured by introducing a sample containing the substrate to be determined into a current of buffer solution, reacting the substrate on an immobilized enzyme and measuring, in a measurement chamber, a physical change in the solution brought about by the reaction, wherein a current of buffer solution containing the sample to be determined is passed alternatingly (i) directly through the measurement chamber with the production of a reference signal and (ii) first over the immobilized enzyme and thereafter through the measurement chamber with the production of a measurement signal, and relating the reference signal to the measurement signal to indicate the enzyme substrate initially present.
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申请公布号 |
US4153513(A) |
申请公布日期 |
1979.05.08 |
申请号 |
US19770826008 |
申请日期 |
1977.08.19 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
EDELMANN, HERMANN;KRAFT, PETER;MANN, KARLHEINZ;SCHULER, PETER;HAGEN, ALEXANDER |
分类号 |
G01N27/02;C12Q1/00;G01N27/416;G01N33/487 |
主分类号 |
G01N27/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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