| 摘要 |
<p>Serological method for determining presence of Neisseria gonorrhoeae antibodies in human sera and products utilized in such testing. The method comprises diluting the serum to be tested in physiological saline at a dilution of about 1 : 2 to 1 : 1000, heating the diluted serum at about 56.degree.C to 65.degree.C for from about 15 to 30 minutes and thereafter incubating with a composition containing a heat labile antigen produced from a growth culture of Neisseria gonorrhoeae to form an antigen-antibody conjugate when said antibodies are present; the antigen being characterized as follows: 1. inactivated by heating at about 56.degree.C for about 30 minutes; 2. inactivated by suspension in an aqueous medium At pH values up to 5; 3. stable in an aqueous medium at pH values of 7 and above; 4. stable when incubated with the following enzymes: deoxynucleic acidase (Enzyme No. 3.1.4.5), ribonucleic acidase (Enzyme No. 3.1.4.22), lipase(Enzyme No. 3.1.1.3), dextranase (Enzyme No. 3.2.1.11), neuraminidase (Enzyme No. 3.2.1.18), lysozyme (Enzyme No. 3.2.1.17), and 5. inactivated when incubated with pronase (Enzyme No. 3.4.24.4), trypsin (Enzyme No. 3.4.21.4) or chymotrypsin (Enzyme No. 3.4.21.1).</p> |
