EASILY SPLIT SUBSTRATES FOR THE QUANTIFICATION OF PROTEASES

摘要

Easily split enzyme substrates for the quantification of proteases having the general formula R1-p-Glu-A1-A2-NH-R2, where R1=H or a protective group, preferably t-butyloxycarbonyl, benzyloxycarbonyl; A1=Gly, Ala, Val, Leu, Ile, Ser, Thr, Pro, Pip, Phe or Tyr; A2=Arg or Lys; R2=an aromatic, possibly substituted, hydrocarbon group, wherein -NH-R2 is a physico-chemically determinable group, preferably a chromogenic or fluorogenic group which is split by a present enzyme and then forms a cleavage product of the formula H2N-R2 the amount of which can be quantified. Processes for the production of said substrates. Method in the laboratory diagnostics of proteases using said substrates.