| 摘要 |
A method for the determination of the quantity and type of a biological component A in a sample which is added to a basin in a liquid-saturated, porous matrix covering a thin layer of a biologically active component B bound to the solid surface of a carrier. A further biologically active component C included in the matrix is capable of reacting biospecifically with component A when the sample is allowed to diffuse or migrate electrophoretically in the matrix forming a zone containing a precipitate of components A and C. This precipitate will adsorb with biospecificity to component B, bound to the solid surface of the carrier and is visualized in a suitable manner on the surface after removal of the said porous matrix, eg. as a change in the surface tension angle observed with the aid of vapour condensation on the surface. Components B and C or B and A may be the same as long as the remaining component is capable of reacting with both the others. The components A, B and C may be antigens and antibodies. The carrier is suitably made of plastic material. |
