| 摘要 |
<p>A gel product for chromatography based on agarose is provided. In addition, a method of using the product for hydrophobe salting out adsorption is also provided. The gel product consists essentially of agarose to which neutral hydrophobic groups which do not form ions are covalently bonded. The hydrophobe groups can be composed of aliphatic hydrocarbons, halogen or hydroxy-substituted alkyl groups, with 1 to 20 carbon atoms, or contain 1 or more, up to 5, aromatic nuclei, unsubstituted or substituted with alkyl, alkyloxy, halogen or NO2 or have the formula where R can be alkyl, alkenyl, aryl, aralkyl, aralkoxy, alkaryl, optionally substituted with hydroxy, halogen or nitro, and where the alkyl parts of R contain 1 to 20 carbon atoms. The method of separating the amphipatic substances is based on the fact that they are adsorbed by an amphipatic gel in which the salt concentration in the solution is preferably adjusted beyond the level at which the ion concentration least corresponds to the ion concentration in an aqueous solution of 1 M NaCl, and desorption takes place through lowering the ion concentration and/or lowering the pH and/or lowering the polarity of the solvent. The amphipatic substances are primarily proteins or peptides.</p> |
