NOVEL RECOMBINANT PLASMID PSG1-12

摘要

PURPOSE:To mass-produce a GRF1-29 derivative by forming a recombinant plasmid pSG1-12 having a DNA sequence coding a peptide fragment of GRF with a fused protein of dihydrofolate reductase. CONSTITUTION:A recombinant plasmid pSG1-12, stably replicative in Escherichia coli, capable of imparting tolerance to trimethoprim and ampicillin to Escherichia coli which is a host, coding a fused protein of a peptide fragment of a growth hormone releasing factor having a specific amino acid sequence and a dihydrofolate reductase and forming a recombinant plasmid and having a specific DNA sequence is formed. The recombinant plasmid encodes DHFR- GRFM and Escherichia coli having the pSG1-12 is capable of accumulating and producing the DHFR-GRFM in a large amount.