| 摘要 |
<p>1534412 Immobilized enzymes OWENSILLINOIS Inc 2 Nov 1976 [15 March 1976] 45442/76 Heading C3H A process for immobilizing a protein on a support to form a biological active composite, comprises the steps of: selecting a protein having the sum total of one-half of the cystine amino acid residues plus the cysteine amino acid residues equalling at least about 14 per mole of said protein, depositing said protein on an inert support to form a protein/support composite, and maintaining said composite at a pH which facilitates polymerization of said protein and maintaining said composite at a temperature and for a time sufficient to polymerize said protein and immobilize said protein in situ on said support. Preferably the protein is an enzyme, e.g. urease. The polymerization process may be effected at pH 3À0 to 6À5, or 9À5 to 11 at 0-20‹ C. The inert support is preferably a refractory oxide powder, e.g. a porous matrix formed by compacting and sintering a refractory oxide powder. Alternatively the support may be in the form of fibres. The enzyme is preferably deposited on the support by soaking said support in an aqueous solution of the enzyme. Examples describe the preparation of urease on alumina.</p> |
