METHOD FOR IMPROVING ORGANISMS USING FLUX SCANNING BASED ON ENFORCED OBJECTIVE FLUX

摘要

A method for improving the productivity of a useful material in a living organism is provided to effectively improve the productivity of the useful material by selecting a gene involving with metabolic pathway between an optimum value and a maximum value of the production-related metabolic pathway from the subject living organism and then introducing it thereinto or amplifying it. The method for screening a gene to be amplified for improving the productivity of a useful material comprises the steps of: (a) after selecting a living organism(except human) which is a subject to produce an object useful material, constructing a metabolic pathway model of the selected living organism; (b) obtaining a maximum value which is a theoretical maximum yield related to the production of the useful material in the constructed metabolic pathway and an optimum value of the production-related metabolic flux of the useful material when the value of the growth related metabolic flux is maximum under the application or the non-application of fermentation data; (c) performing an FSEOF(flux scanning based on enforced objective flux) algorithm between the optimum value and the maximum value of the metabolic path obtained from the step(b) to make out a profile of total metabolic flux of the metabolic pathway; (d) if the maximum among the relative values of the total metabolic flux values in the profile is larger than the optimum value, selecting a gene involving with metabolic flux as a first gene to be amplified; and (e) determining a gene involving with the metabolic flux showing the simple increase or the simple decrease from the gene selected from the step(d) as a final gene to be amplified. To improve a living organism producing a useful material, the final gene obtained from the step(e) is introduced to a subject living organism or the gene is amplified in the subject living organism.