IMMOBILIZED BIOLOGICALLY ACTIVE SUBSTANCE AND THE USE THEREOF

摘要

1533579 Immobilized biologically active substance I G H SJOHOLM N R LINDMARK and B M EKMAN 15 Oct 1975 [16 Oct 1974 (3)] 42219/75 Heading C3H An immobilized, biologically active substance comprises spherical, microporous particles consisting of a three-dimensional polymeric network, wherein said particles have a diameter of less than 10 Ám. and an average diameter of 0À5 to 4 Ám., said biologically active substance being entrapped in the meshes of the network. The particles preferably consist of a copolymer of an acrylic compound of not more than 10 carbon atoms or of 1-vinyl-2-pyrrolidone, with a divinyl compound of not more than 10 carbon atoms. The particles most preferably consist of a copolymer of N,N1-methylenebis-(acrylamide) and CH 2 = CR1-COR 2 wherein R 1 is H or CH 3 , R 2 is H or NHR 3 (wherein R 3 is H, C 1 -C 6 alkyl in which the alkyl is optionally substituted by hydroxyl or oxo). The biologically active substance may be labelled with an atom or atom group which emits radiation, with an enzyme or with a chromogen. In the Examples, the polymer matrix is polyacylamide or acrylic acid/N,N1-methylenebis- (acrylamide), and the biologically active substance is albumin; Concanovalin optionally modified with fluorescienisothiooyanate; human serum albumin modified with fluoresceinisothiocyanate / Concanovalin; protein A from Staphylococcus aureus; glycine bonded to dextran modified with cyanogen bromide, to which insulin or I125-insulin is bonded; bromosulphophthalein or polyethyleneglycol modified therewith; poly-1-lysine, serum albumin; I125- modified albumin; ferratin-modified albumin; helix pomalia agglutin; iodomodified polytyrosine, helix pomatia agglutinin coupled to collagen; rabbit antimorphine serum; rabbit antidigitoxin antibodies; guinea pig antiinsulin serum; protein from Staphylococcus aureus; dinitrophenyl modified bovine immunoglobulin G; globulin from human blood serum.