摘要 |
The extraction of ligninolytic enzymes by cultivation of whiterot fungi can be improved in that at the point of incipient colouration at least 0.1 g/l, in particular 0.3 to 5 g/l, and especially 1 g/l of manganese dioxide powder, in particular finely divided powder with a grain size of less than 10 .mu.m, is added to the fungal culture and the pH value in the medium is kept constant at a value between 4.8 and 5.0 during the whole of the culturing period. It is preferred that the enzymes be periodically separated off from the culture medium by ultrafiltration and that the residual culture substrate be recycled.
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