A CRYOPRESERVATION DEVICE AND METHOD

摘要

A device and method suitable for the cryopreservation of all types of bio logical cells is described. In this method, an ultra-fast cooling/warming de vice system is used to achieve vitrification of individual cells or cell sus pensions without cryoprotectant agents (CPA) or with a low concentration of CPAs (<1M), to attenuate the formation of intracellular ice crystal forma tion during cooling, and to minimize devitrification during subsequent warmi ng. The device system applies oscillating heat pipe (OHP) and nanofluid tech niques, and is built through microfabrication. Several devices may be networ ked to increase the total volume of cell samples that the cryopreservation s ystem can process simultaneously.